Yeast sugar and balloon practical

I am about start a yr9 practical, yeast , sugar and water placed in a test tube produces CO2 , and is supposed to inflate a balloon placed over the neck of the test tube. However hardly any inflation is visible. Does anyone have any tips ? Thank you.
 
Gave out dried yeast and glucose and let them mix it in the boiling tube with warm water. Left in a warm water bath. Start at the beginning of the lesson to give it a lot of time. Most classes got some good results.
 
Let them set it up in 100ml conicals and "leave in the lab" until next lesson. Before next lesson, add a dash of ethanol to each conical, chill in fridge, put balloon back on, allow to warm to room temperature in time for them to view. If the heating is on, put them on/near the heater because biological molecules work best if they're kept warm.
 
Not sure a test tube sized sample would inflate a balloon
That said....
You need to jump start the yeast
Mix up your yeast suspension about an hour prior to the lesson and pop it into a water bath at around 35oC
About half hour before the lesson sprinkle around 1-2g of glucose over the surface of the yeast suspension
By the time the lesson starts it'll be frothing like mad as the yeast respires
 
Not sure a test tube sized sample would inflate a balloon
That said....
You need to jump start the yeast
Mix up your yeast suspension about an hour prior to the lesson and pop it into a water bath at around 35oC
About half hour before the lesson sprinkle around 1-2g of glucose over the surface of the yeast suspension
By the time the lesson starts it'll be frothing like mad as the yeast respires
it will make it stand up, it's a standard fermentation practical.
 
it will make it stand up, it's a standard fermentation practical.
I know that
I've just always seen it done on a larger reaction vessel - conical flask, 500ml or bigger plastic bottle etc

Never on a test tube
 
This have never worked and yeast is expensive so I give out test tubes and balloons but inflate the balloons for the next time they're in science. Ive got some really funny shaped balloons with funny colours too lol so they love it
 
I am about start a yr9 practical, yeast , sugar and water placed in a test tube produces CO2 , and is supposed to inflate a balloon placed over the neck of the test tube. However hardly any inflation is visible. Does anyone have any tips ? Thank you.
Is it the respiring yeast practical? You've got to heat the water up to 30ºC (a kettle works for that). Stir the mixture.
 
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We used to either arrange this for the summer/early autumn or put it over the radiator/in water baths depending on what they were investigating. But it was pretty messy and temperamental.
 
I am about start a yr9 practical, yeast , sugar and water placed in a test tube produces CO2 , and is supposed to inflate a balloon placed over the neck of the test tube. However hardly any inflation is visible. Does anyone have any tips ? Thank you.
I have been researching this and found this information. We now put two spatulas of yeast into a test tube and add 7ml of glucose solution. We get a result in the 50degrees water bath
 
I think the trouble is usually, here at least, is not doing this at the beginning of the lesson. When experiments are rushed in the last 10-15 minutes of the lesson it is little wonder the results are rubbish.
 
I think the trouble is usually, here at least, is not doing this at the beginning of the lesson. When experiments are rushed in the last 10-15 minutes of the lesson it is little wonder the results are rubbish.
of a double and the teacher phones at the start asking where the practical is..............
 
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