One agar plate disposal (no autoclave bags available)

Greetings to everyone,

I am going to lock down today the prep room for a long time period,

There is one agar plate with a bacteria culture in that is left for disposal,

The thing is that I can't autoclave it because I don't have autoclave bags,

Can I use ethanol instead? If yes which type of method? Just embedding the agar plate in ethanol?

Regards
 
i'd still email cleapss to check as this isn't my area of expertise, but i would say put what you need to dispose of into a bag, tape it closed, label it and put in it the freezer. It should at least stop any growth.

But I would definitely email CLEAPSS before doing it.
 
i'd still email cleapss to check as this isn't my area of expertise, but i would say put what you need to dispose of into a bag, tape it closed, label it and put in it the freezer. It should at least stop any growth.

But I would definitely email CLEAPSS before doing it.
cleapss say you can leave it in a bag in the prep room, no need to freeze it.

If you don’t have time to also sterilise inoculated plates leave them taped shut in an open autoclave bag on the side in a locked prep room (avoid leaving on a windowsill). These will remain safe to handle for a considerable amount of time. Sterilise these as soon as possible once you have access to school again.
 
cleapss say you can leave it in a bag in the prep room, no need to freeze it.
Even easier then.

I was lucky and hadn't started making my plates up for the a-level micro PAG so didn't have to worry about disposal!
 
Greetings to everyone,

I am going to lock down today the prep room for a long time period,

There is one agar plate with a bacteria culture in that is left for disposal,

The thing is that I can't autoclave it because I don't have autoclave bags,

Can I use ethanol instead? If yes which type of method? Just embedding the agar plate in ethanol?

Regards
Sorry bit late to the question but just in case anyone else needs to do the same. Until we invested in a load of autoclave bags, we used roasting bags available from any supermarket. Ideal for a few plates.
 
Sorry bit late to the question but just in case anyone else needs to do the same. Until we invested in a load of autoclave bags, we used roasting bags available from any supermarket. Ideal for a few plates.

Thanks Old Dog, actually I was told exactly that by CLEAPSS Helpline
 
I'd agree with those that said just freeze it till you do a batch of autoclaving. They're fine in the freezer for years. Put it in a bag or cling film it first.
 
I have a couple of agar plates in my fridge awaiting autoclaving and they have lost all their liquid and turned into very thin 'poppodoms'! They will be okay in there till I get round to getting rid!
 
the whole petri dish without opening it, put the bag in the autoclave, then open/pull the bag down to allow the steam in
 
the whole petri dish without opening it, put the bag in the autoclave, then open/pull the bag down to allow the steam in

I am not quite sure that I have understood what do you mean,

Maybe because I never used an autoclave bag before
 
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