Gram staining

[Micro23]

I have a gram staining practical soon. I have 1 % crystal violet solution. Is this a good concentration to use or would it be better diluted to 0.5%? Many thanks

 

[chris everitt]

1. Dissolve 2 g crystal violet in 20 ml of 95% ethyl alcohol.
2. Dissolve 0.8 g ammonium oxalate monohydrate in 80 ml deionized water.
3. Mix the crystal violet and ammonium oxalate monohydrate solutions to make the crystal violet stain. Filter the stain if necessary.

This works for our Biologists, hope it helps

 

[Micro23]

Thank you for the reply . I have the 1 percent aqueous solution, can this be diluted down with distilled water?