Sudan III

Discussion in 'Supporting Biology' started by j_a18, Jun 20, 2019.

  1. Hi, i am new to the technician role and have been asked to prepare Sudan III without any guidance. I was wondering if I could get some help on how to make It? I have the powder and I understand it can be mixed with ethanol or water. I opted for water to save costs. It doesn’t seem to be dissolving at all in the water. I followed a procedure I saw online as can’t find how to make it up in CLEAPSS. Can someone confirm the quantity with water? I went off 10grams per 100ml. Is this correct?

    Thanks in advance
    Snailo likes this.
  2. If you go back in Supporting Biology area, there is a post from the beginning of June re: Sudan III. Basically, it won't dissolve in water, it needs to be ethanol.
    Dave T likes this.
  3. ClaireS and Snailo like this.
  4. The thing is J_a18 that if you use the powder you will only need to use a grain of it to test for lipid.:)