Practical Microscopy - Stomata

Discussion in 'Supporting Biology' started by GeorgetheScienceTech, Sep 10, 2018.

  1. Here is a pic of Tradescantia Stomata using a Green Plastic Filter. The photo was taken directly down the Eye Piece of the Microscope.

    Psomata 002.jpg
     
  2. Nice pic George. Is the green filter in the eyepiece or is it on the microscope stage? Also, is the particular shade of green critical?
     
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  3. Hi diving belle The stage. The shade of Green is not critical. I found out by experimentation that the use of a Green Filter seemed to intensify the outline of the Stomata for this particular plant (one which is frequently used in A level Biology). Oh well, that's another giveaway from my book.:D:D:D
     
    Last edited: Sep 10, 2018
  4. Most excellent George, really does show off the outline (and excellent photography too!)

    I know you said it's frequently used in A lvl Biology, but I personally prefer this to the stomata 'peel' for KS3, feels a little more real.

    Here's my attempt without a filter directly down the microscope for comparison.

    Tradescantia.jpg
     
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  5. Excellent! Some really good detail A Walter.:);)
     
  6. Carol Taylor

    Carol Taylor Footsore

    I'll pass that one on to my biologists.
     
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  7. I found that Tradescantia Zebrina (purple type) can be viewed down an ordinary light microscope with no filters, no stain and using the whole leaf - just cut a square, place it in slide, add a drop of water and place coverslip on top.

    The leaf is thin enough to let light through and the stomata do not have the purple colouring in them so they stand out. You can even do an osmosis experiment with this by placing squares of leaves in differing concentrations of sugar then observing down the microscope to see which have open/closed stomata. You can then estimate the osmotic potential of the guard cells.
     

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  8. A PE/Biology Teacher Carol?:D:D
     
  9. Nice Pic Peter. Are you sure you haven't used a Green Filter or is it that the Stomata of this Zebrina species are actually Green?:);)
     
  10. The guard cells are green.
     

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  11. I'm looking to get myself some of these plants, it'll make that lesson so much easier.

    Do you just cut a bit off the leaf and put it on a slide? Someone once told me you had to peel the leaf?

    Are the plants easy to care for?
     
  12. Yes you do need to peel the leaf and but a small portion on the slide. the plants are quite expensive but easy to maintain and the cuttings will quickly root.:);)
     
  13. I just took a leaf that fell off in transit and plonked it under the microscope (bottom side up!) It was a sunny day so no additional lighting at all. No peeling, no cutting, no cover slip, nothing. Hence why I like it for KS3 - to try and build that enthusiasm that they can go and grab something and look at it under the microscope.
     
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  14. Well done you!:);)
     
  15. No you don't need to peel the leaf, it is thin enough to let light through to illuminate the stomata - The image I posted has no stain, no filter, no varnish - just a square piece raw leaf under a coverslip with a drop of water.

    I bought 4 cuttings from ebay for £3.99 - they rooted in water and I transferred them to normal compost - They are incredibly easy to grow and maintain.
    https://www.ebay.co.uk/itm/Wanderin...003408?hash=item4d7d05a3d0:g:A90AAOSw2DdbMVY5
     
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  16. I just tried a white and green striped one - the only one we had - no coverslip or peeling - well impressed. I'm going to bring a purple one in from home!
     
  17. Carol Taylor

    Carol Taylor Footsore

    HAHAAHA, no a real one who is actually a triple specialist.