ONPG

Discussion in 'Supporting Biology' started by greenfingers, Apr 10, 2019.

  1. greenfingers

    greenfingers Sarah G

    Hi
    Does anyone know how to make up this for B galactosidase test using colorimetry.TIA
     
  2. Interesting request greenfingers. ONPG Aka o-nitrophenyl-beta-D-galactopyranoside. I'm assuming that you are doing gene induction or perhaps a simple Discontinuous (Deadstop) enzyme assay. I'm afraid that you will have to buy this substrate for Beta-Galactosidase in as manufacturing this is beyond the capabilities of us mere mortals. :);)This may help:

    https://www.chem-agilent.com/pdf/strata/200383.pdf
     
  3. greenfingers

    greenfingers Sarah G

    thanks George