Discussion in 'Supporting Biology' started by greenfingers, Apr 10, 2019.
Does anyone know how to make up this for B galactosidase test using colorimetry.TIA
Interesting request greenfingers. ONPG Aka o-nitrophenyl-beta-D-galactopyranoside. I'm assuming that you are doing gene induction or perhaps a simple Discontinuous (Deadstop) enzyme assay. I'm afraid that you will have to buy this substrate for Beta-Galactosidase in as manufacturing this is beyond the capabilities of us mere mortals. This may help:
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