Biurets

Discussion in 'Supporting Biology' started by Sciencey Sue, Oct 11, 2018.

  1. Can anyone advise me on the concentrations of copper sulphate and sodium hydroxide for the Biurets test? CLEAPSS recommend 0.01M CuSO4 and 0.1M NaOH. Although this gives a colour change in the presence of protein it is from blue to a deep purple. We can't get the pinky-lilac colour that is indicated on Wikipedia. :confused:
     
  2. No worries! We've sorted it!! We were using 0.1M CuSO4 instead of 0.01M, the bluer colour of which turned our lilac to a deep purple!! :rolleyes:
     

  3. Easily done!