Biuret test - how much egg white?

Discussion in 'Science Technicians - General Discussion' started by Gill T, Mar 13, 2018.

  1. Hello all,
    I've been asked to make up an "egg white solution" for the biuret test for protein. Any suggestions for the egg white to water ratio? I was thinking 1 egg white in 250ml water. Sound about right?
    Yours hopefully,
    Gill
     
  2. Should be about 1%w/w Gill. It won't do any harm to thow 5 eggs in the mix - it will give a more intense colouration. Remember to use Free Range eggs.:D:D:D
     
  3. Buy in egg albumen powder. 1% is fine.
     
    Claire and Elizabeth Gould like this.
  4. Yes, but...needed tomorrow, of course.
     
  5. I boil the kettle, put about 600ml in a beaker, stir fast on a hotplate and add one egg-white.
     
  6. We do two egg whites to 700ml of water. Heat over a Bunsen stirring all the time until the temp reaches 70 deg C then turn off the heat
     
  7. I probably wouldn't heat the Albumin as it would then not be soluble and you need soluble protein for the Biuret. Even when you buy in Albumin powder it takes a while for the protein to be hydrated. Good luck.:);)
     
    Claire likes this.